Composite

Part:BBa_K1326003

Designed by: Leah Simmons   Group: iGEM14_Macquarie_Australia   (2014-10-08)

Plac + POR + DVR1 + ChlP + ChlG

Composite part entailing genes at the end of the chlorophyll biosynthetic pathway, responsible for the construction of the chlorophyll molecule. Light-dependent protochlorophyllidereductase (POR), a chloroplast precursor, uses NADPH and light to convert protochlorophyllide to chlorophyllide. DVR1 is a chloroplast transit peptide. Plant geranylgeranylhydrogenase (ChlP) reduces free geranylgeranyldiphosphate to phytildiphosphate; this leads to the construction of the chlorophyll side-chain Chlorophyllsynthetase (ChlG) is a catalyst for the esterification of chlorophyllide with phytyl-pyrophosphate to make the chlorophyll molecule.

PSB1C3 Operon 3.png
Figure 1: Operon 3 in the chlorophyll biosynthesis pathway.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 808
    Illegal BglII site found at 4090
    Illegal BglII site found at 4579
    Illegal BamHI site found at 1571
    Illegal BamHI site found at 3859
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 347
    Illegal AgeI site found at 398
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2461


Functional Parameters

GEL Operon 3 Chlorophyll a.png
Figure 1: Single and double restriction digests of Operon 3. Total plasmid length is 7.10 kB, as can be seen by the dark band in the single digest lane. Double digest lane shows the operon fragment at 4.90 kB, and the BioBrick plasmid backbone at 2.20.

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